FOX-04 - Forkhead Box Protein

Forkhead Box Protein 4‑D‑Retro‑Inverso (FOX‑04 / FOXO4‑DRI) is a synthetic senolytic peptide engineered to help selectively neutralize and remove senescent cells — aged or damaged cells that persist in tissues, contribute to chronic inflammation, impair recovery, and accelerate aging.

By targeting the interaction between FOXO4 and p53, FOX‑04 facilitates programmed apoptosis of senescent cells, supporting tissue renewal, improved cellular environments, and research into longevity and anti‑aging pathways.

Key Features

🧪 cGMP manufactured in an FDA‑registered facility
✅ ≥98% purity confirmed by HPLC analysis
📄 Batch‑specific Certificates of Analysis (COAs) included
💉 Sterile, lyophilized vials for research use only

Human‑Relevant Research Applications

⚡ Selective senescent cell apoptosis (senolysis)
FOX‑04 peptide disrupts the FOXO4–p53 interaction, triggering p53‑mediated apoptosis selectively in senescent cells — a key mechanism underlying cellular senescence research.
🔗 https://pubmed.ncbi.nlm.nih.gov/28340339/

🧬 Targeting cellular aging pathways
Designed FOXO4‑based peptides have been shown to act as senolytic agents in human cell cultures by releasing p53 from senescent cell nuclei, enabling the removal of dysfunctional cells.
🔗 https://pubmed.ncbi.nlm.nih.gov/40739602/

📊 Reduced senescence markers in human models
FOXO4‑derived peptides like FOXO4‑DRI have demonstrated the ability to reduce senescent cell burden in human chondrocytes and other cell models, providing a translational research tool for studying aging and tissue quality.
🔗 https://pubmed.ncbi.nlm.nih.gov/33996787/

Mechanism Summary

• Disrupts FOXO4–p53 binding: Releases p53 from senescent cell nuclei, enabling apoptosis of aged/damaged cells.

• Selective senolytic action: Targets cells with high FOXO4 expression while sparing normal proliferating cells.

• Supports tissue homeostasis research: Clearing senescent cells can improve cellular environments for regeneration and reduce pro‑inflammatory signaling in experimental models.

Reconstitution & Handling Guidance

1️⃣ Use sterile bacteriostatic water (0.9% benzyl alcohol)
2️⃣ Add 3 ml of bacteriostatic water to reconstitute the peptide
3️⃣ Gently swirl until fully dissolved (do not vortex)
4️⃣ Store reconstituted peptide at 4 °C–10 °C for 2–3 months
5️⃣ Unreconstituted peptide can be stored at 4 °C–10 °C until the expiration date

Legal & Research Disclaimer

This material is supplied exclusively for laboratory research use. It is not intended for diagnostic, therapeutic, cosmetic, human, or veterinary applications. The purchaser assumes all responsibility for compliance with applicable local, state, and federal regulations governing research compounds.